英语翻译1.measure out 5% less distilled water than desired total

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英语翻译
1.measure out 5% less distilled water than desired total volume of medium.Using a mixing container that is as close to the final volume as possible.
2.Add powdered medium to 15 to 30℃ (room temperature) water with gentle stirring.(Do not heat water)
3.Rinse out inside of package to remove all traces of powder.
4.When completely dissolved.adjust ph to 4.4.2 with 1N HCL.
5.Dispense 95 ml in 100ml bottles.Do not cap tightly.
6.Autolave for 15min at 121℃.Exhaust on slow cycle .
7.Allow to cool to room temperature.
8.Add 3 ml.Sterile 7.5% NahCO2 solution to each bottle.Mix well.
9.Add sterile L-glutamine and antibiotic solutions serum or other supplements as desired.Mix well.
10.Adjust to final PH 7.2-7.4 with 1N NaOH or HCL if necessary.

1.量取比需求的媒质总容量少5%的蒸馏水.用一个尽量与总体积接近的搅拌容器.
2.将粉质媒介加入15到30摄氏度（室温）的水中轻轻搅拌（水不要加热）.
3.将容器内部的粉末清洗干净.
4.彻底溶解后,用1N 盐酸将pH值调至4.4.2.
5.用100ml瓶子配制95ml.不要盖得太紧.
6.在121摄氏度下用热压处理15分钟.低速运转排气.
7.允许冷却至室温.
8.在每个瓶中加入3毫升7.5%的无菌NahCO2溶液.充分搅拌.
9.根据要求加入无菌的L-谷氨酰胺,抗体溶液血清或其他代替品.充分搅拌.
10.如果需要,用1N NAOH或HCL 将最后pH调至7.2-7.4.

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