搜搜考试网英语翻译The human embryonic kidney cell line HEK293 was obtained
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英语翻译
The human embryonic kidney cell line HEK293 was obtained from the American Type Culture Collection (Manassas,VA,USA).Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supple- mented with 10% fetal bovine serum (FBS; Hyclone,Logan,UT,USA).Human recombinant EGF,U0126,SB203580,and SP600125 were purchased from Calbiochem (San Diego,CA,USA).Antibodies against Egr-1 and GAPDH were obtained from Santa Cruz Biotechnology (Santa Cruz,CA,USA).The firefly and Renilla Dual-Glo(TM) Luciferase Assay System was purchased from Promega (Madison,WI,USA).
The human embryonic kidney cell line HEK293 was obtained from the American Type Culture Collection (Manassas,VA,USA).Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supple- mented with 10% fetal bovine serum (FBS; Hyclone,Logan,UT,USA).Human recombinant EGF,U0126,SB203580,and SP600125 were purchased from Calbiochem (San Diego,CA,USA).Antibodies against Egr-1 and GAPDH were obtained from Santa Cruz Biotechnology (Santa Cruz,CA,USA).The firefly and Renilla Dual-Glo(TM) Luciferase Assay System was purchased from Promega (Madison,WI,USA).
人类胚胎肾细胞系HEK293获得了美国菌种保藏中心（马纳萨斯,弗吉尼亚州,美国）.细胞保持贝科的改良Eagle的中期（培养液）,柔软,有10％胎牛血清（胎牛血清,胎牛血清,洛根,UT斯达康,美国）mented.重组人表皮生长因子,U0126,SB203580和SP600125购自Calbiochem（圣地亚哥,加利福尼亚,美国）.对废气再循环抗体- 1和GAPDH的,是根据生物圣克鲁斯（圣克鲁斯,CA,美国）.萤火虫和海肾双辉牌（商标）萤光素酶检测系统是购自试剂盒（麦迪逊,威斯康星州,美国）.
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